The most powerful woman on Wall Street is tapping the biggest banks to back her new tech venture, The Post has learned.

Blythe Masters, a former commodities bigwig at JPMorgan Chase, is looking to raise $25 million or more in the first round of funding for Digital Asset Holdings — and she’s aiming at her former peers to back her venture, informed sources say.

Masters’ company uses the bitcoin-like technology called “blockchain” to shave millions of dollars in costs at banks by making settlement and record-keeping more automated.

Competition has heated up among financial companies for being the first to adopt the cutting-edge technology and be seen as an “industry leader.”

The $25 million funding round, which is the first for the company, is ongoing, according to one insider familiar with the fundraising effort.