The feds today took a second bite at the millions in ill-gotten gains allegedly accumulated by Bernard Madoff’s former longtime office manager.

Annette Bongiorno is now on the hook for more than $5 million, with prosecutors saying they’ve found more than $2 million worth of additional assets since first filing suit against her in June.

The updated Manhattan federal court filing targets a luxury, Colonial-style home on Long Island that Bongiorno and her husband bought for $1.4 million in 2000, and a lakeside home at a Boca Raton, Fla., country club for which they paid $862,000 in 1995.

The suit also seeks forfeiture of a 2007 Mercedes-Benz E550 sedan, worth $65,900, that Bongiorno purchased in 2007. All the loot will be auctioned off to pay back victims of Madoff’s record $68 billion Ponzi scheme, the Manhattan US Attorney’s Office said.