The Federal Aviation Administration issued an order on Tuesday night that barred all US planes from entering the airspace below 26,000 feet over Venezuela as the South American country braced for a second day of political violence.

The FAA told air operators to refrain from entering Venezuela’s airspace until further notice, citing the country’s “increasing political instability and tensions.”

Any planes in Venezuela, including private jets, should leave the country within 48 hours, the statement read.

According to the air traffic website FlightAware, the airspace over Venezuela was occupied by a single Iberia flight headed from Colombia to Madrid, Spain late Tuesday night.

Venezuela’s opposition leader Juan Guaido urged supporters to launch a new round of mass protests on Wednesday as beleaguered president Nicolas Maduro attempted to hold onto power.