WASHINGTON — Harvard University is engaging in unlawful racial and sexual discrimination and prioritizing at least $49 million in taxpayer-funded DEI programming, according to a civil rights complaint filed by a conservative legal group Wednesday.

America First Legal (AFL), along with the Department of Justice’s Office of Civil Rights, claimed that the Ivy League school was violating Title VI and Title IX, along with President Trump’s executive actions and the US Supreme Court’s 2023 ruling outlawing race-based affirmative action.

Some of the alleged violations were made possible due to the closure of Harvard’s Office for Equity, Diversity, Inclusion and Strategic Belonging and opening of the Office for Community and Campus Life — one of many “rebranding” moves that have kept the “substance of its discriminatory DEI practices,” AFL attorney Megan Redshaw wrote in the complaint letter.

Others have been carried on through internship, clerkship and Harvard Medical School’s residency pipelines, Redshaw’s letter noted.

Among the outlays cited in the complaint is a $21.9 million grant for Harvard Medical School’s Clinical and Translational Science Center to “train and diversify” its workforce by prioritizing “underrepresented” and “diverse” populations to address “health inequities.”

At least $8.9 million in federal grant funding was approved for doctoral and medical programs calling DEI a “core priority” to recruit “young talent.”

The feds also awarded another $1.7 million grant for a study on “climate factors, racial/ethnic disparities, and menstrual cycle health.”

And a $1.3 million grant went to “culturally sensitive” interventions for “climate justice” in “marginalized and at-risk communities” worldwide.

The US Department of Health and Human Services, whose civil rights office already faulted Harvard for “deliberate indifference towards harassment of Jewish and Israeli students,” greenlit the funding among the millions of dollars in grants handed out since 2021 to the Cambridge, Mass., institution.

Those outlays have since been blocked by the Trump administration as it seeks to end tolerance of antisemitism, race-based discrimination and DEI initiatives on campus.

Though it had informed Harvard of its nondiscrimination obligations, the US Department of Education has also been barred from enforcement due to preliminary injunctions from federal courts.

AFL suggested in its 26-page letter laying out the case for further investigation to Assistant Attorney General Harmeet Dhillon that Harvard’s accreditation could still be in jeopardy.

It also noted that the Ivy League university still uses “diversity-based essay prompts” in its admissions, pointing to a video posted publicly in May sharing some examples.

“Rather than dismantle its ‘discrimination, exclusion, and intolerance’ practices as required, Harvard has chosen instead to rename, repackage, and redeploy the same unlawful practices under new euphemisms,” the group’s letter stated. “It has chosen defiance over compliance.”

America First Legal demanded “a formal investigation into Harvard Medical School’s admissions, scholarships, clerkships, residency pipelines, affiliated residency programs, faculty hiring, and research practices,” according to the complaint.

It also asked for another look at the “rebranding scheme,” “pipeline and recruitment initiatives” and called for the dismantlement of “all DEI infrastructure.”

The DOJ declined to comment. Harvard reps did not immediately respond to a request for comment.