A group of Long Island parents and educators is suing state Attorney General Letitia James for muzzling them on trans-student-athlete issues — while forcing officials to call kids by their preferred pronouns.

The complaint, filed in federal court Tuesday, takes issue with James’ “guidance letter” to school boards in May cautioning members that discussing student trans issue at public meetings could violate youngsters’ right to privacy — and warning them that if they do so, “they may be removed” by state education officials.

“They want to threaten your seat every time you don’t fall in line,” raged Kerry Wachter, president of the Massapequa School Board and one of the plaintiffs, to The Post, referring to state education officials. “That’s why we’re a target.

“I think they use it as an intimidation factor for everything,” Wachter said of the threats. ” ‘Do what we say, or we’re going to take your seat.’ That’s kind of how this state runs. So this is the heavy-handedness of New York state education and the attorney general.”

The lawsuit — filed by the Southeastern Legal Foundation on behalf of Wachter, Danielle Ciampino, a member of the Rotterdam-Mohonase Central School District, two Rockville Centre Union Free School District and others — takes issue with James’ May 25 warning on discussing student trans issues.

“Some board members have made, and encouraged, comments during board meetings that demean and stigmatize LGBTQ+ students,” the letter said. “These comments have included attacks on school support for LGBTQ+ student groups and on transgender and gender-expansive students’ rights to use facilities.”

It added among its directives, “Nor should boards allow individuals to intentionally misgender district students–a practice that, according to research, leads to feelings of stigmatization and emotional harm, including anxiety, depression, and stress.’’

The letter concludes with an ominious reminder.

“Board members may be removed by the commissioner of education if they, (1) violate the education law or another law ‘pertaining to [public] schools,’ including the state Human Rights Law; (2) willfully neglect their duties as public officers; or (3) willfully disobey a ‘decision, order, rule or regulation’ of the Regents or the commissioner of education,” the missive said.

The suing school-board members and parents maintain the AG is trying to stifle discussion on an important issue that has affected students, including female Massapequa students who raised concern at one meeting about biological males sharing their locker room.

“I’m here to talk about a statement that was made at the last meeting that the problem with the voice changing in the girls locker room was a problem for the parents,” a teen said at a recent meeting.

“I’m here to say, as a female student, it’s hard enough to change in front of my female classmates, never mind if we add voice to the situation,” the girl said.

Trans issues have been a hotly debated topic in several Long Island communities, including the controversial ban on transgender athletes at county sports facilities passed by the Nassau County legislature in June.

James’ office declined to comment to The Post on the lawsuit Tuesday, while describing the May 25 letter as just an explanation of state laws and guidelines.