Dozens of firefighters and their loved ones ran from Long Island to Ground Zero Wednesday to honor their 343 FDNY brothers lost on Sept. 11, 2001.

It took nearly nine hours for the dedicated group of 15 runners — carrying massive American flags and flanked by 50 others on bikes — to complete the grueling 34.3-mile journey through three boroughs and a sliver of Long Island.

“As we come up West Street and see the Freedoms Tower with half a mile remaining all the pain in the legs suddenly becomes negligible and people cheering us on really brings tears to my eyes,” organizer and firefighter Etan Blatt told The Post, adding that the experience brought a “wave of emotion.”

“To see the support for our endeavor grow each year means everything to me because our primary mission is and will always remain, honoring the 343 FDNY members that were killed that day 23 years ago and as we run through the streets of NY with our group and our flags held high, certainly reminds people of the importance of this day.”

The run marked the fourth annual event by the 34.3 Foundation, a charity that benefits the firehouses and families of FDNY officers killed in the line of duty.

The dedicated group of active firefighters and their loved ones wearing matching red T-shirts started in Long Beach at 8:47 a.m. — exactly one minute after observing a moment of silence for the first plane strike into the North Tower of the World Trade Center complex.

Fifty others followed closely behind on bikes, carrying crucial nutrition, water and emotional support.

The dedicated group wearing matching red T-shirts started in Long Beach at 8:47 a.m. — exactly one minute after observing a moment of silence for the first plane strike into the North Tower of the World Trade Center complex.

Fifty others followed closely behind on bikes, carrying crucial nutrition, water and emotional support.

They stopped several times at firehouses along the way — including for five moments of silence to honor major milestones in the national tragedy: for the Flight 175, 77 and 93 crashes and for the collapse of both the North and South Towers.

They even hauled a wooden American flag placard, which they bestowed onto Hook and Ladder Company 3 in Union Square — just four miles out from the finish line at One World Trade Center.

“The guys are doing great, we’re working as a team. The younger guys are pushing the younger guys for sure,” Blatt said as he chronicled the arduous, day-long journey on social media.

The now-annual run — 30% longer than a marathon — was born in 2021 when Blatt, a seasoned runner, came up with a plan to mark the 20th anniversary of the terror attacks, which killed 2,977 people.

He had no intention of starting a charity, until he lost friend and fellow firefighter Jesse Gerhard the following year.

“It shined a light on how much support firehouses and the families of the fallen really need financially and emotionally,” said Blatt.

“Our goal was to make sure the family knows they are cared for and know that we don’t want them to worry about putting all those things on their credit cards. We want them to worry about taking care of each other and grieving and between not only us, but other organizations like ours along with the FDNY ceremonial unit, they certainly feel taken care of.”

Blatt founded the 34.3 Foundation as a means to raise funds throughout the year for FDNY firehouses and families suffering line-of-duty deaths. It offers financial support for housing costs for families, food and funeral expenses.

This summer, the group raised an estimated $20,000.

Several other firefighters joined Blatt for the demanding run the next two years, but the event boomed in its fourth iteration.

“To see how much support we’ve received in 3 years is amazing and somewhat overwhelming,” said Blatt.