It’s last call for a famed Queens gin joint.

Open since 1977, the Sly Fox Inn — across from St. John’s University — has been a favorite bar for generations of students and basketball stars, including coach Lou Carnesecca and players Chris Mullin, Anthony Mason Jr. and Metta World Peace.

Owner Ed Moore, 61, says the $20,000-per-month rent became too much to handle.

Last call is set for June 27.