The estranged husband of missing Connecticut mother Jennifer Dulos was arrested Wednesday and charged for a second time with tampering with evidence in connection with her disappearance, police said.

Connecticut State Police took Fotis Dulos, 51, into custody at his home around 2:30 p.m. and brought to Troop G in Bridgeport, police said.

He was released on $500,000 bond Wednesday evening, police said.

That’s the same amount he was freed on June 11 after he and his girlfriend Michelle Troconis, were arrested earlier that month on charges of tampering with evidence and hindering prosecution.

Prosecutors alleged the pair dumped several trash bags containing items soaked with Jennifer’s blood in the hours following her disappearance on May 24.

Both Fotis and Troconis pleaded not guilty to those charges.

Authorities didn’t immediately provide details on the new charge. Dulos’ lawyers didn’t respond to requests for comment. Dulos’ lawyer Norm Pattis said he intends to plead not guilty at his next hearing, Sept. 12 in Norwalk Superior Court.

“It is difficult to see why the state waited for months to serve this warrant. It was unnecessary,” Pattis said in a statement. “We wish the state police spent more time looking for Jennifer and less trying to build a case against Fotis.”

At the time of Jennifer’s disappearance, she and her real estate developer husband were embroiled in a nasty divorce battle.

The 50-year-old homemaker from a wealthy family was last seen dropping off their children at school the morning of her disappearance.

Later, she missed two appointments in New York City and her car was found abandoned on a New Canaan road.

The next day, cops found “multiple stains” on the floor and a car inside the garage at Jennifer’s New Canaan home that “tested positive for human blood,” court documents showed.

There were “multiple areas of blood splatter’’ — and “evidence of attempts to clean the crime scene,’’ the papers said.

“Investigators came to the consensus that a serious physical assault had occurred at the scene, and Jennifer Dulos was the suspected victim,’’ the court affidavit said.

The night she went missing, a man matching Dulos’ description in a truck like the one he owns, was seen leaving the vehicle during stops “and placing multiple garbage bags into various trash receptacles.”

Some of Jennifer’s clothing, including a T-shirt and bra, and household goods like sponges stained with her blood were recovered from the trash cans, authorities said.

Since Jennifer’s disappearance, her mother, Gloria Farber, has been caring for her five children and is seeking full custody.

Their dad has been duking it out with Farber over custody of the kids, who he hasn’t seen since his June arrest.

He recently requested that his mother-in-law undergo a psychiatric evaluation, citing alleged past mental health issues.

Farber is also suing Dulos in civil court for $2.5 million, claiming he failed to repay business loans his real estate development company, Fore Group, took out.