The Brooklyn federal judge who as a prosecutor​ won a conviction against the late Gambino boss John Gotti​, ending his run as the “Teflon Don,” is resigning from the bench, officials said Monday.

John Gleeson is stepping down after serving 21 years as judge in the Eastern District, Chief Judge Carol Bagley Amon confirmed.

“The court is losing a very distinguished judge and a valued colleague,” Amon said in a statement.

Gleeson’s longtime career in law was marked by putting the ​Gotti away for life on murder and racketeering charges in 1992 while a prosecutor with the US Attorney’s Office.

He used mob hitman Sammy “The Bull” Gravano’s testimony to bury Gotti in a cooperation agreement that landed Gravano just five years behind bars for 19 murders.

​Gotti died of cancer in prison.​

Gleeson, a dad of two, was appointed as a federal judge in 1994 by former President Bill Clinton.

He didn’t immediately return messages.