The Treasury Department sanctioned 328 members of the Russian Duma — as well as the Duma itself — on Thursday as President Biden met in Belgium with the leaders of NATO countries to plot the latest moves responding to Russian President Vladimir Putin’s month-old invasion of Ukraine.

The Treasury Department said in a statement that four dozen companies linked to Russia’s defense sector — including conglomerate KTRV — and the CEO of Russia’s largest bank were among entities also facing new sanctions.

“This action aligns with similar actions taken by the European Union, the United Kingdom, and Canada, and reflects continued unity to hold Putin accountable for his war of choice,” the department said.

A dozen Duma members had already been sanctioned by the US earlier this month, bringing the penalized total to 340 members of the 450-strong lower house of Russia’s parliament. Putin’s United Russia party controls 325 seats.

The Treasury cited the Duma members’ support for Russian recognition of the independence of a pair of pro-Moscow separatist states in eastern Ukraine, which served as a pretext for the invasion that began Feb. 24.

In its statement, the department described the recognition of the self-proclaimed Donetsk People’s Republic and Luhansk People’s Republic as a violation of “core principles of international law, such as respect for the territorial integrity of other states.”

The new penalties were rolled out as the war enters its second month.

Sanctions generally authorize the seizure of assets of targets and hinder their ability to conduct international business.

The Treasury Department said that “[b]y cutting off 48 companies from western technological and financial resources, today’s action will have a deep and long-lasting effect on Russia’s defense-industrial base and its supply chain.”

Russia is a major arms exporter to other countries.

Throughout the conflict, Biden has faced broad bipartisan pressure from Congress to do more to penalize Russia and support the government of Ukrainian President Volodymyr Zelensky.

Biden announced initially limited sanctions against state-owned Russian banks and certain Russian businessmen after the invasion began. After criticism, Biden sanctioned Putin’s vast personal wealth and reached an agreement with US allies to partially unplug Russia from the SWIFT international banking system.

As Congress readied legislation to force his hand, Biden announced an import ban on Russia oil, natural gas and coal. Also in response to bipartisan pressure, he banned imports of Russian alcohol, diamonds and seafood and said he would move to strip Russia of it “most favored nation” trade status, allowing high tariffs on other goods.

Biden has also imposed limits on certain technology exports to Russia and applied sanctions to a growing list of Russia’s elite — though for now Russia’s richest woman, billionaire Yelena Baturina, who allegedly paid $3.5 million in 2014 to a company linked to first son Hunter Biden, has not yet faced sanctions.

Most US companies have also pulled out of Russia, including Visa, MasterCard and McDonald’s.